Gull gull IFN-gamma gene and application of recombinant protein coded by same

• Main Authors: HU ZHIHUI, REN SHENGJIE, RUAN QIAN, ZHU MAOYIN, YANG LINFU, XIANG XUN, DUAN BOFANG, DAI FEIYAN, DUAN GANG, WU JIALI, CHANG HUA
• Format: Patent
• Language: Chinese; English
• Published: 14.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to application of a red-mouth gull IFN-gamma gene and a recombinant protein coded by the red-mouth gull IFN-gamma gene, and belongs to the field of biology. The method comprises the following steps: firstly, finding a gull gull IFN-gamma gene, then recombining the gull gull IFN-gamma gene with a pET32a (+) vector to obtain a prokaryotic expression plasmid, transforming the recombinant prokaryotic expression plasmid into Transsetta (DE3) expression bacteria, adding an IPTG inducer to induce protein expression, purifying to obtain a recombinant protein, and carrying out purification by using the recombinant protein to obtain the gull gull IFN-gamma gene. After the Newcastle disease virus is co-incubated with the gull gull lymphocytes respectively, the related gene transcription level is detected, and after the recombinant protein and the Newcastle disease virus (NDV) are co-incubated with the gull gull lymphocytes, the related gene transcription level is detected, which shows that after th