Novel self-assembled porcine coronavirus nanoparticle vaccine and application thereof

• Main Authors: LI GUOXIN, SONG JUNHAN, WANG YUANHONG, YU LINGXUE, TONG GUANGZHI
• Format: Patent
• Language: Chinese; English
• Published: 14.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a novel self-assembled nanoparticle vaccine for porcine delta coronavirus and an application of the novel self-assembled nanoparticle vaccine. In this study, the immunogenicity of ferritin nanoparticles coupled to RBD dimers has been designed and evaluated. The RBD-Fe induces to generate a large number of RBD specific antibodies and can also induce to generate high-level neutralizing antibodies, and Kunming mice are protected from obvious PDCoV infection symptoms after being attacked by viruses. Meanwhile, the RBD-Fe vaccine not only can induce generation of CD3 + CD19 + B cell populations, but also can induce high-percentage THT and CTL cell populations, which indicates that the nanoparticle vaccine also can activate powerful T cell immune response and eliminate possibly infected cells. 本发明提供一株自组装的猪δ冠状病毒新型纳米颗粒疫苗及其应用心。在本研究中，设计并评估了与RBD双聚体偶联的铁蛋白纳米颗粒的免疫原性。RBD-Fe诱导产生大量的RBD特异性抗体，也可诱导产生高水平的中和抗体，并保护昆明小鼠在病毒攻击后无明显PDCoV感染症状。同时，RBD-Fe疫苗不仅可诱导产生CD3+CD19+B细胞群，同时也诱导了高百分比的THT及CTL细胞群，这表明纳米颗粒疫苗也能够激活强大的T细