Probe-based nucleic acid and protein analysis

• Main Authors: HILL ADRIAN J, PFEIFFER KATHERINE, KOWE, ANDREW, S, LUND PAUL EUGENE
• Format: Patent
• Language: Chinese; English
• Published: 07.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for treating a biomolecule can include hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), and barcoding the probe-nucleic acid molecule complex or derivative thereof. Such methods may include performing nucleic acid reactions, such as extension, denaturation, and amplification. Methods for processing a sample can include hybridizing a probe to (i) a target region of a nucleic acid molecule (e.g., an RNA molecule) and (ii) a reporter oligonucleotide of a characteristic binding group, and barcoding the molecule associated with the probe. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. 本文提供了用于处理来自样品的生物分子(例如，核酸分子、蛋白质)的系统和方法。用于处理生物分子的方法可以包括使探针分子与核酸分子(例如，核糖核酸(RNA)分子)的靶区域杂交，并为所述探针-核酸分子复合物或其衍生物加条形码。这种方法可以包括进行核酸反应，例如延伸、变性和扩增。用于处理样品的方法可以包括使探针与(i)核酸分子(例如，