Preparation method of high-temperature yeast seed fungicide

The invention provides a preparation method of a high-temperature Daqu seed fungicide, and relates to the technical field of bioengineering and wine brewing. A preparation method of the seed fungicide is a freeze-drying method and at least comprises the following steps: preparing a microbial liquid...

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Bibliographic Details
Main Authors ZHANG JIABIN, LU YUJIE, LI HONGMEI, HOU YAOCHUAN, LIU QINGSONG, ZHANG KAIZHENG
Format Patent
LanguageChinese
English
Published 07.11.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
OTHER ALCOHOLIC BEVERAGES
PREPARATION THEREOF
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a preparation method of a high-temperature Daqu seed fungicide, and relates to the technical field of bioengineering and wine brewing. A preparation method of the seed fungicide is a freeze-drying method and at least comprises the following steps: preparing a microbial liquid which comprises 21 high-temperature yeast functional microorganisms, and centrifugally concentrating the seed bacterial liquid into a high-concentration bacterial liquid; crushing and sieving bran, rice husks and corncobs, mixing according to a certain proportion to serve as a microbial carrier, and mixing with the concentrated bacterial liquid; the added protective agent comprises the following components in parts by weight: 5-8 parts of trehalose, 4-7 parts of mannitol, 6-9 parts of sodium thiosulfate and 8-10 parts of skim milk powder. According to the method, the thalli are subjected to adaptive vitrification pre-freezing in a manner of freezing at-20 DEG C and then putting into liquid nitrogen, so that the mic
Bibliography:Application Number: CN202311024708