Method for constructing animal model of central nervous immune cell preparation

• Main Authors: LU, MINGQI, YOU YA
• Format: Patent
• Language: Chinese; English
• Published: 07.11.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; NEW BREEDS OF ANIMALS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; TRAPPING

The invention provides an animal model construction method of a central nervous immune cell preparation. The animal model construction method comprises the following steps: S1, selecting a target animal and a homologous close parent of the target animal; s2, carrying out immune cell separation, transformation, amplification and purification on the homologous close parent of the target animal according to the operation specification of an immune cell therapy, so as to obtain an immune cell preparation from the homologous close parent; and S3, injecting the homologous close immune cell preparation into the cerebrospinal fluid of the target animal in an intrathecal injection manner to obtain the animal model of the central nervous immune cell preparation. According to the animal model construction method, immune cells required by separation and purification of homologous close-parent mice are adopted, after modification, cerebrospinal fluid is directly injected by bypassing a blood brain barrier in an intratheca