Rapid three-dimensional light sheet fluorescence microscopic imaging device and method based on light field regulation and control

• Main Authors: YAO BAOLI, MIN JUNWEI, CHIYOSHI, YU XIANGHUA, YANG RUIWEN, YANG YANLONG
• Format: Patent
• Language: Chinese; English
• Published: 03.11.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS; TESTING

The invention discloses a rapid three-dimensional light sheet fluorescence microscopic imaging device and method based on light field regulation and control, and relates to the technical field of optical instruments. The imaging device comprises a laser light source module, an illumination light field regulation and control module, a galvanometer scanning light sheet generation module, an illumination and detection orthogonal double objective lens module, a sample pool module, a detection light field regulation and control module and a wide field imaging module which are sequentially arranged along a light path, the system further comprises a control and data acquisition module. According to the invention, by regulating and controlling the point spread function of the detection imaging system, the imaging depth of field can be expanded, clear imaging in a large axial area can be realized, delay of mechanically moving a detection objective lens or a sample is avoided, and three-dimensional microscopic imaging