Recombinant saccharomyces cerevisiae strain with high yield of cannabinoid phenolic acid as well as construction method and application of recombinant saccharomyces cerevisiae strain

• Main Authors: GAO PEIZHEN, GUO JIULONG, LUO XIAOZHOU, ZHANG YUNFENG, YAN WEI
• Format: Patent
• Language: Chinese; English
• Published: 03.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of metabolic engineering, in particular to a recombinant saccharomyces cerevisiae strain with high yield of cannabinoid phenolic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae strain. According to the invention, a beta-oxidation pathway gene POX1 is firstly knocked out, so that the problem of degradation of a hexanoic acid substrate is solved; then, expression of OA pathway enzyme TKS and/or OAC is enhanced, an expression promoter of fatty acid synthetase FAS1 is replaced, and the conversion rate of a hexanoic acid substrate is increased; the gene INO2 related to endoplasmic reticulum membrane synthesis is further expressed, the cannabinoid phenolic acid synthetase gene SOD1-tCsPT4n or CUP1-tCsPT4n containing the fusion protein is expressed, and the synthesis efficiency of cannabinoid phenolic acid is improved. Finally, the expression level of part of genes in saccharomyces cerevisiae cells is regulated and control