Application of two-photon excitation fluorescence combined second harmonic imaging technology in noninvasive living body detection of animal hair regeneration

• Main Authors: WANG FENGLONG, HE GAIYING, WANG YI, LIU MENGHUA
• Format: Patent
• Language: Chinese; English
• Published: 03.11.2023
• Subjects: DIAGNOSIS; HUMAN NECESSITIES; HYGIENE; IDENTIFICATION; MEDICAL OR VETERINARY SCIENCE; SURGERY

The invention discloses application of a two-photon excitation fluorescence combined second harmonic imaging technology in noninvasive living body detection of hair regeneration. Experiments prove that on the premise that no fluorescent substance is marked, the change of hair trunks and hair follicles directly related to hair regeneration in the complete growth cycle of mice is directly, noninvasively and in vivo studied through the two-photon excitation fluorescence imaging technology and the second harmonic imaging technology; and further evaluating important characteristics of hair regeneration and different stages of a growth cycle. The invention provides an important technology and method for reducing the dosage of experimental animals, screening hair regeneration drugs and performing non-invasive evaluation on hair regeneration on human scalp. The method has an important application value. 本发明公开了双光子激发荧光联合二次谐波成像技术在无创活体检测毛发再生中的应用。实验证明，本发明在不标记任何荧光物质的前提下，通过双光子激发荧光成像技术和二次谐波成像技术直接无创、活体研究小鼠完整生长周期内与毛发再生直接相关的毛干和