Vaccine for preventing and treating potato virus Y and preparation method thereof

• Main Authors: WU ZHAOYUN, XU SHIXIAO, GUO YUGE, LI JUNYING, ZHOU FANG, YANG TIEZHAO, HE BING, ZHOU JIANFEI, SUN JUTAO
• Format: Patent
• Language: Chinese; English
• Published: 31.10.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a vaccine for preventing and treating potato Y virus (PVY), which comprises pTRV1 and at least one recombinant expression vector pTRV2-G-target gene, the target gene is PVY-HC or PVY-NIb, and the sequences of the target gene are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. When the vaccine is used on tobacco, a buffer solution is added into the vaccine to activate the vaccine, and the vaccine and the buffer solution are mixed uniformly; storing in a shading manner at room temperature, adding water into the obtained mixed solution, and then uniformly spraying the mixed solution onto tobacco seedlings with cut leaves according to the spraying amount that water drops visible to naked eyes exist on leaf surfaces. The bacterial liquid can effectively silence exogenous invaded potato Y virus gene expression, the gene silencing efficiency is up to 54.7%, propagation of the potato Y virus in a pla