Endoclita serpta chitin synthase 1 (PeCHS1) gene, dsRNA thereof, synthesis method and insect resistance application of endoclita serpta chitin synthase 1 (PeCHS1) gene

• Main Authors: ZHAN YAGUANG, JING TIANZHONG, QI FENGHUI, ZENG FANSUO, WANG WENXUAN
• Format: Patent
• Language: Chinese; English
• Published: 31.10.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an endoclyta lophila chitin synthase 1 (PeCHS1) gene, dsRNA thereof, a synthetic method and insect resistance application thereof.The endoclyta lophila chitin synthase 1 (PeCHS1) gene and dsRNA thereof are characterized in that a specific primer pair of an open reading frame of the PeCHS1 gene is designed and synthesized through transcriptome analysis, a PeCHS1 gene sequence is obtained through gene cloning, the nucleotide sequence is SEQ ID NO: 1, the amino acid sequence is SEQ ID NO: 2, then a gene segment with the sequence being SEQ ID NO: 3 is selected as an RNAi target sequence, the RNAi target sequence is selected as a nucleotide sequence, and the dsRNA is selected as a dsRNA target sequence. The method comprises the following steps: designing and synthesizing primer pairs SEQ ID NO.6 and SEQ ID NO.7 with enzyme cutting sites, carrying out PCR amplification, constructing a prokaryotic expression vector, and inducing dsRNA expression. DsRNA feeding is carried out on the endoclyta