Single cell sorting micro-fluidic chip and single cell sorting method

• Main Authors: GUAN YIMIN, WANG KUN, YANG MIAOMIAO
• Format: Patent
• Language: Chinese; English
• Published: 31.10.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention provides a single cell sorting micro-fluidic chip and a single cell sorting method.The chip comprises a cell supply tank, a micro-column array and a plurality of discrete capture units, the cell supply tank is divided into an injection area and an outflow area through the micro-column array, the multiple discrete capture units are connected with the outflow area, and the injection area is connected with the injection area through the micro-column array. Each capturing unit is provided with a respective liquid flow driving part, so that the liquid flow of each capturing unit can be independently controlled, and the situation that the overall flow resistance is remarkably increased along with the increase of the number of the capturing units is avoided, so that the number of the capturing units is not limited or is less limited, and higher flux is realized. By matching the flow resistance proportion of the capturing channel and the bypassing channel, the single cells can be easily intercepted, and