Specific molecule identification method for ganoderma heavy umbrella

• Main Authors: LUO TIANTIAN, TAN JIENING, LI XIUNIAN, TANG YINBIN, JIANG GUOFENG, LIU SISI, HUANG ZIZHAO, HUANG YONGQI, WU XINLING, XU LIMING, LIU CHANG, QIN FENGHAI, QIN LIUTING
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a specific molecular identification method for ganoderma lucidum, and belongs to the technical field of molecular biology, and the method comprises the following steps: obtaining a specific primer combination F/R of a ganoderma lucidum conserved nucleotide sequence molecular marker, extracting a to-be-detected ganoderma lucidum sample DNA as a template, respectively carrying out PCR amplification reaction by using the specific primer combination F/R, a reaction product is detected through agarose gel electrophoresis, and a sample with a specific band of an F/R primer amplification product is ganoderma heavy umbrella ganoderma. The invention provides a group of molecular markers, namely a specific nucleotide sequence of ganoderma atrum, and the specific nucleotide sequence can be used for distinguishing ganoderma atrum from other species of ganoderma lucidum, so that identification, protection and development of ganoderma atrum are facilitated. 本发明提供一种用于重伞灵芝的特异性分子鉴别方法，属于分子生物学技术领域，所述方法包括如