Preparation method of carboxymethyl chitosan immobilized enzyme based on charge selectivity

The invention discloses a charge selectivity-based carboxymethyl chitosan immobilized enzyme preparation method, which comprises: (1) adding a natural positively or negatively charged enzyme to a nano-silica aqueous solution, violently mixing, and carrying out oscillation treatment so as to complete...

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Bibliographic Details
Main Authors WANG WEI, HUANG WENCAN, MAO XIANGCHAO
Format Patent
LanguageChinese
English
Published 27.10.2023
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Summary:The invention discloses a charge selectivity-based carboxymethyl chitosan immobilized enzyme preparation method, which comprises: (1) adding a natural positively or negatively charged enzyme to a nano-silica aqueous solution, violently mixing, and carrying out oscillation treatment so as to completely adsorb the enzyme to the surface of the nano-silica; (2) adding positively or negatively charged carboxymethyl chitosan into the solution, violently mixing, and oscillating; and (3) removing the nano silicon dioxide template, and washing with a buffer solution to obtain the immobilized enzyme. Carboxymethyl chitosan with different charge characteristics is adopted as a material, carboxymethyl chitosan with phase reaction charge characteristics is selected, and carboxymethyl chitosan and enzyme are self-assembled on the surface of nano silicon dioxide through electrostatic interaction; the supramolecular self-assembly immobilized enzyme with high specificity, high activity and general applicability is prepared, t
Bibliography:Application Number: CN202311155097