Salmonella enteritidis ryhB-1 and stdA double-gene deletion strain as well as construction method and application thereof

• Main Authors: WANG HENG, WEI HUAXU, MENG XIA, ZHU GUOQIANG, HE MENGPING, XIA PENGPENG, ZHANG KE
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of bioengineering, and particularly relates to a salmonella enteritidis ryhB-1 and stdA double-gene deletion strain SE50336 [delta] ryhB-1 [delta] stdA as well as a construction method and application thereof. The double-gene deletion strain disclosed by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20221958. The deletion strain is obtained by knocking out non-coding small RNA (Ribonucleic Acid) RyhB-1 and stdA genes of the salmonella enteritidis 50336 by utilizing a lambda-Red homologous recombination technology. The virulence of the double-gene-deleted strain prepared by the invention is obviously reduced, and the double-gene-deleted strain has good safety. The avian salmonella enteritidis attenuated vaccine can induce relatively strong cellular immunity and humoral immunity, has a good challenge protection effect and an excellent immune effect, can be eliminated within 3-4 weeks, has excell