Chicken infectious laryngotracheitis subunit vaccine and application thereof

• Main Authors: DU YUYIN, GUO WEIWEI, LIU DAWEI, XIANG YINHUI, CUI XIAOXIA, CHU DIANFENG, DU YUANZHAO
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a chicken infectious laryngotracheitis subunit vaccine, and belongs to the technical field of animal immune drugs. The chicken infectious laryngotracheitis subunit vaccine provided by the invention is prepared from chicken infectious laryngotracheitis virus gD protein coded by a nucleic acid molecule of an optimized chicken infectious laryngotracheitis gD gene, tetanus toxin T cell epitope peptide Q-Y-I-K-A-N-S-K-F-I-G-I-T-E is specifically introduced into an amino acid sequence of the gD protein, and the tetanus toxin T cell epitope peptide Q-Y-I-K-A-N-S-K-F-I-G-E is specifically introduced into the gD protein. The 205th amino acid is mutated into A from N. The vaccine does not contain genetic materials, has no toxin expelling and dispersing risks, has a better cellular immune effect, and can be effectively applied to challenge protection of the avian infectious laryngotracheitis virus strain. 本发明提供了一种鸡传染性喉气管炎亚单位疫苗，属于动物免疫药物技术领域。本发明提供的鸡传染性喉气管炎亚单位疫苗，采用优化后的鸡传染性喉气管炎gD基因的核酸分子编码的鸡传染性喉气管炎病毒gD