E.coli residual total RNA (Ribonucleic Acid) detection kit and use method thereof

• Main Authors: ZHANG DAN, XU JINCHAO, LI HONGJIAN, ZHANG WEINAN, GUO BINBIN, CAI YUQING, LI YONGFENG, LIU LIMEI
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biology, in particular to an E.coli residual total RNA (Ribonucleic Acid) detection kit and a use method thereof, and the kit comprises an E.coli RNA quantitative standard substance, One Step Enzyme Mix and E.coli RNA PrimerMix. The amplification efficiency of the E.coli residual total RNA detection kit provided by the invention is 90-110%, the stability is good, and the detection result of a sample is more reliable. 本发明涉及生物技术领域，尤其涉及一种E.coli残留总RNA检测试剂盒及其使用方法，所述试剂盒包括E.coli RNA定量标准品、One Step Enzyme Mix、E.coli RNA Primer⪻Mix。本发明提供的E.coli残留总RNA检测试剂盒的扩增效率在90-110％，稳定性好，样本的检测结果更加可靠。