Kit and method for qualitatively detecting ureaplasma urealyticum RNA in human urine sample, male urethral swab and female cervical swab

• Main Authors: CHEN YUENING, LI SHAN, DONG ZIWEI, LIU YUE, JIANG XIWEN, XU RENCHAO, HUANG ZHIWEN
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The embodiment of the invention belongs to the field of pathogen molecule detection, and relates to a kit and a method for qualitatively detecting ureaplasma urealyticum RNA in a human urine sample, a male urethral swab and a female cervical swab, the kit comprises a UU RNA detection reagent, a UU RNA digestion reaction solution, a UU RNA internal standard solution and a UU RNA quality control product, the UU RNA quality control product comprises a UU RNA positive quality control product and a UU RNA negative quality control product, the UU RNA detection reagent comprises a first reaction reagent, a second reaction reagent and a third reaction reagent, and the first reaction reagent comprises a UU RNA upstream primer, a UU RNA downstream primer, a UU RNA probe, an internal standard upstream primer, an internal standard downstream primer and an internal standard probe. The method has the advantages of few false positives, short detection period and the like. 本申请实施例属于病原体分子检测领域，涉及一种定性检测人尿液样本、男性尿道拭子、女性宫颈拭子中解脲脲原体R