Paris polyphylla mosaic necrosis virus infectious clone vector with GFP gene and construction method of paris polyphylla mosaic necrosis virus infectious clone vector

• Main Authors: LUO HENGMING, TAN GUANLIN, LAN PINGXIU, CHEN XIAOJIAO, LI FAN, HE PENG
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a paris polyphylla mosaic necrosis virus infectious cloning vector with a GFP gene and a construction method of the paris polyphylla mosaic necrosis virus infectious cloning vector, and belongs to the field of plant virology and genetic engineering. According to the paris polyphylla mosaic necrosis virus infectious cloning vector with the GFP gene, a yeast recombination method is utilized, a cDNA full-length sequence corresponding to PMNV whole genome RNA is cloned between a double 35S promoter and a terminator NOS of a pCB301-HDV-2 vector, and a green fluorescent protein reporter gene is inserted between an NIb protein and a CP protein of PMNV, so that a pCB301-PMNV-GFP recombinant vector is obtained. According to the invention, a PMNV infectious cloning vector constructed by using a yeast homologous recombination technology is the first infectious clone of a plant virus on a Pseudomonas aeruginosa, and the constructed pCB301-PMNV-GFP vector can stably, efficiently and systematically