Preparation method of MOFs encapsulated enzyme for separation and analysis of low-molecular-weight protein

• Main Authors: YUAN XIAOYU, SONG JIAYI, SU PING, YANG YI, SONG HANYUE, ZHANG WENKANG
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a preparation method of an MOFs encapsulated enzyme for separation and analysis of low-molecular-weight protein, and belongs to the technical field of immobilized enzyme preparation. The surface of trypsin (Try) is modified with polyacrylic acid (PAA) to synthesize a Try-PAA compound, the Try-PAA compound and a ZIF-L precursor (zinc nitrate hexahydrate and 2-methylimidazole) aqueous solution are incubated together, Zn < 2 + > is attracted through PAA which is crosslinked with Try and has negative electricity, and thus Try is packaged in ZIF-L. According to the method, the defect of low efficiency is overcome, the Try is packaged in the ZIF-L in situ through a biomimetic mineralization method, and Try leakage is effectively prevented. The prepared hydrophobic mesoporous Try-PAA-ZIF-L is applied to enzymolysis analysis of LWPs in a model protein mixture and a human serum sample, and shows excellent size selective enzyme digestion performance. The preparation method is mild in preparation