Subtilase mutant and application thereof

• Main Authors: BIAN FURONG, BAI AIXI, LI HUANHUAN, XU YUANXI, DU LIJUAN, WANG XUEJIAO
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

Provided herein is a subtilisin mutant comprising one or more of the following amino acid substitutions: S3T, N43R, N76D, P129Q, V205I, Q206L, Y209W, S259D, N261W and L262E compared to a wild type subtilisin, in which: 1) the position of the amino acid substitution corresponds to the position of the amino acid sequence as shown in SEQ ID NO: 1; 2) the subtilisin mutant has protease activity; and 3) the amino acid sequence of the wild subtilisin is selected from the amino acid sequence shown as SEQ ID NO: 2 or 4. Also provided herein are detergent compositions comprising the subtilisin mutants and uses of the subtilisin mutants. Compared with the existing product, the subtilisin mutant provided by the invention has the advantages that the storage stability is improved, the use of a protease stabilizer is avoided, and the production cost of the subtilisin mutant is reduced. 本文提供了枯草杆菌酶突变体，与野生型枯草杆菌酶相比，其包括如下氨基酸取代中的一个或更多个：S3T、N43R、N76D、P129Q、V205I、Q206L、Y209W、S259D、N261W和L262E，其中：1)所述氨基酸取代的位置对应于SEQ ID NO:1所示氨基酸序列的位