Complex enzyme preparation rich in high-enzyme-activity saccharifying enzyme and feruloyl esterase as well as strain and application of complex enzyme preparation

• Main Authors: HAN YEDONG, SUN HONGHAO, LYU FUJUN, ZHOU YUMENG, CHEN XIUXIU
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention belongs to the technical field of fermentation engineering, and discloses a solid complex enzyme preparation containing high-enzyme-activity saccharifying enzyme and feruloyl esterase as well as a strain and application thereof. In the compound enzyme preparation disclosed by the invention, the enzyme activity of glucoamylase is 80000 to 100000U/g, the enzyme activity of feruloyl esterase is 500 to 600U/g, the enzyme activity of acid protease is 22000 to 25000U/g, the enzyme activity of neutral protease is 500 to 700U/g, the enzyme activity of pectinase is 2000 to 3000U/g, the enzyme activity of xylanase is 900 to 1000U/g, the enzyme activity of cellulase is 100 to 200U/g, the enzyme activity of beta-mannase is 100 to 200U/g, and the enzyme activity of galactosidase is 50 to 60U/g. The preparation method of the solid compound enzyme preparation comprises the following steps: preparing a spore suspension from aspergillus niger subjected to slant culture, adding the spore suspension into a culture