Acipenser Yangtze River primer, probe and detection method based on environmental DNA (Deoxyribose Nucleic Acid)

• Main Authors: CHEN SHASHA, LI ZEHAO, HE WENPING, ZHANG LIANBO, CAO MINGSONG, YAO WEIZHI, HU JIAWEN
• Format: Patent
• Language: Chinese; English
• Published: 20.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an acipenser Yangtze River primer based on environmental DNA, a probe and a detection method. The nucleotide sequence of an upstream primer CJX-12s-F2 is as shown in SEQ ID No.1, the nucleotide sequence of a downstream primer CJX-12s-R2 is as shown in SEQ ID No.2, and the nucleotide sequence of a probe CJX-P2 is as shown in SEQ ID No.3. The invention further provides a kit for detecting the acipenser Yangtze River primer based on environmental DNA. And a real-time fluorescence PCR detection method for the acipenser Yangtze River is established, so that the aim of accurately identifying the acipenser Yangtze River is fulfilled. The method can be applied to qualitative detection of the Yangtze River sturgeons in the water body only by collecting a water sample of a water area in which the Yangtze River sturgeons live, and is high in specificity and good in timeliness; the density of the Yangtze River sturgeons in the environment can be quantitatively detected, and the biomass and time-spa