Primer and kit for early screening of bladder cancer and application

• Main Authors: WANG SHUPENG, ZENG QINLONG, XIA LU, FANG HU, WU SONG, LEI QIFANG
• Format: Patent
• Language: Chinese; English
• Published: 20.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides primers and a kit for early screening of bladder cancer and application of the primers and the kit, suspicious high-frequency bladder cancer gene segments are screened out through an algorithm, and methylation primers of all target gene segments are designed according to MethPrimers. DNA in T24 bladder cancer cells is extracted, methylation treatment is carried out, the experiment process is simulated, and the feasibility and accuracy of the designed primer are verified. 8 pairs of primers and a pair of internal reference primers with relatively high sensitivity and specificity are determined; according to the present invention, 100 samples (70 bladder cancer patients and 30 normal persons) are subjected to urine DNA extraction, methylation treatment, qPCR quantitative treatment and regression analysis, such that the value of the confirmed primer in bladder cancer diagnosis is verified; the obtained primer is stable and reliable, and has high sensitivity and specificity through joint de