CRISPR/Cas9-based detection test strip method for gastric cancer related LncRNA HOXA10 and application of CRISPR/Cas9-based detection test strip method

• Main Authors: JIANG HAN, WANG HUA, HU ANLA, ZHAO QIHONG, QIN PANZHU
• Format: Patent
• Language: Chinese; English
• Published: 13.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biomedicine, and provides a rapid detection method of gastric cancer related LncRNA HOXA10 in a clinical sample, a primer pair aiming at the specificity of the gastric cancer related LncRNA HOXA10 is shown as SEQ ID NO.1 and SEQ ID NO.2, CRISPR/Cas9 comprises Cas9 protein and sgRNA, the sequence of the sgRNA is shown as SEQ ID NO.3, and the sequence of a gold probe is shown as SEQ ID NO.4. A lateral flow chromatography technology comprises a supporting plate, a detection platform, a detection platform and a detection platform, and the detection platform is used for detecting the gastric cancer related LncRNA HOXA10 in the clinical sample. The upper end face of the supporting plate comprises a sample loading area, a lineation area and a water absorption area which are sequentially arranged, a detection line T combined with streptavidin and a quality control line C of a capture probe are arranged in the lineation area, and the sequence of the capture probe is as s