Method for improving Sox2 expression level of DP cells
The invention belongs to the technical field of cell culture, and particularly relates to a method for improving the Sox2 expression level of DP cells. The DP cells are separated by adopting a method comprising the following steps: cleaning a hair follicle sample; removing at least part of fat and c...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
13.10.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention belongs to the technical field of cell culture, and particularly relates to a method for improving the Sox2 expression level of DP cells. The DP cells are separated by adopting a method comprising the following steps: cleaning a hair follicle sample; removing at least part of fat and connective tissue, segmenting the remaining hair follicle sample, and collecting the part containing DP cells to obtain DP cell segment tissue; adding enzymatic hydrolysate into the collected DP cell segment tissues for enzymolysis, so that DP cells are exposed; stopping enzymolysis, repeatedly blowing and beating to enable the DP cells to fall out from the DP cell segment tissues, and centrifuging; and carrying out solid-liquid separation to obtain the DP cells, and cleaning the obtained DP cells. Compared with the same-generation passage cells obtained by passage of DP cells separated by adopting a microdissection method, the passage cells obtained by performing adherent culture on the primary DP cells separated b |
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Bibliography: | Application Number: CN202310865494 |