Method for improving Sox2 expression level of DP cells

The invention belongs to the technical field of cell culture, and particularly relates to a method for improving the Sox2 expression level of DP cells. The DP cells are separated by adopting a method comprising the following steps: cleaning a hair follicle sample; removing at least part of fat and c...

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Bibliographic Details
Main Authors HAN DONGMEI, FANG PANFENG, YU YU, HUANG YINGZHI
Format Patent
LanguageChinese
English
Published 13.10.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention belongs to the technical field of cell culture, and particularly relates to a method for improving the Sox2 expression level of DP cells. The DP cells are separated by adopting a method comprising the following steps: cleaning a hair follicle sample; removing at least part of fat and connective tissue, segmenting the remaining hair follicle sample, and collecting the part containing DP cells to obtain DP cell segment tissue; adding enzymatic hydrolysate into the collected DP cell segment tissues for enzymolysis, so that DP cells are exposed; stopping enzymolysis, repeatedly blowing and beating to enable the DP cells to fall out from the DP cell segment tissues, and centrifuging; and carrying out solid-liquid separation to obtain the DP cells, and cleaning the obtained DP cells. Compared with the same-generation passage cells obtained by passage of DP cells separated by adopting a microdissection method, the passage cells obtained by performing adherent culture on the primary DP cells separated b
Bibliography:Application Number: CN202310865494