Method for preparing polysaccharide-protein conjugate

• Main Authors: WINTERS MICHAEL ALBERT, BAJAJ JITENDRA, WEN EMILY, HE JUN
• Format: Patent
• Language: Chinese; English
• Published: 13.10.2023
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The present invention provides a method for preparing a polysaccharide-protein conjugate, in which the polysaccharide-protein conjugate is prepared under conditions that improve conjugation reaction consistency, increase protein consumption during the conjugation reaction, produce a higher molecular weight conjugate, and/or reduce the content of free cyanide in the conjugation reaction product. Polysaccharides, typically from bacteria, are conjugated to a carrier protein by reductive amination. The polysaccharide-protein conjugates obtained using these methods can be used for inclusion in multivalent vaccines. 本发明提供了制备多糖-蛋白质缀合物的方法，其中在改善缀合反应一致性、增加缀合反应期间蛋白的消耗、产生更高分子量的缀合物和/或降低缀合反应产物中游离氰化物的含量的条件下，通常来自细菌的多糖通过还原胺化与载体蛋白缀合。使用这些方法获得的多糖-蛋白缀合物可用于包含在多价疫苗中。