MiRNA marker for early pregnancy diagnosis of cattle and application of miRNA marker

• Main Authors: WANG XINGPING, JI ZHONGXIANG, LUO RENG ZHUOMO, WANG JINPENG, BAO BINWU, YANG YI
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a miRNA marker for early pregnancy diagnosis of cattle and application of the miRNA marker, and belongs to the technical field of biology, the miRNA marker is selected from any one or two of bta-miR-133a and bta-miR-19b, early pregnancy of the cattle is rapidly diagnosed by detecting the expression quantity of the miRNA in blood of the tested cattle, and the miRNA marker can be applied to early pregnancy diagnosis of the cattle. When the expression quantity of the bta-miR-133a in the blood of the tested cattle is obviously reduced or the expression quantity of the bta-miR-19b is obviously increased, or the expression quantity of the bta-miR-133a is obviously reduced and the expression quantity of the bta-miR-19b is obviously increased, it is prompted that the tested cattle is in the early stage of pregnancy. Therefore, any one or two of the bta-miR-133a and the bta-miR-19b can be used as a novel marker for the early pregnancy diagnosis of the cattle, and is used for the early pregnancy