Nucleic acid constant-temperature detection method by combining T7 endonuclease I with lateral chromatography

• Main Authors: WANG YAO, ZHANG KUNPENG, ZHANG WENHUI, WENG SHAOTING
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to a nucleic acid detection method, which comprises the following steps: amplifying target nucleic acid of a to-be-detected sample, combining with the target nucleic acid through a specific double-labeled DNA probe, the DNA probe is a sequence complementarily paired with a specific site of the target nucleic acid, and has 2 or 3 bases not paired with the target nucleic acid inside, and when the target nucleic acid exists in the to-be-detected sample, the target nucleic acid in the to-be-detected sample is not paired with the target nucleic acid. The DNA probe is combined with target nucleic acid, T7 endonuclease I can specifically recognize two or three non-paired base sites of the DNA probe in a binding area and cut open, the broken double-labeled DNA probe does not develop color on a detection line of the corresponding lateral chromatography test paper, and when a sample to be detected does not contain target nucleic acid, the DNA probe cannot be effectively combined, so that the targe