Method for producing L-hydroxyproline through biological conversion

• Main Authors: BA HONGJUAN, ZHANG MENGJIAO, PANG YIGENG, LI QIAOLI, TIAN CHAOBO
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biological engineering, and discloses a method for producing L-hydroxyproline through biological conversion, which comprises the following steps: preparing a culture medium from glucose, ammonium sulfate, yeast powder, monopotassium phosphate and other trace elements, performing expanding culture on genetically engineered bacteria, feeding the genetically engineered bacteria into a tank in a stress manner, performing online feeding of ammonia water and 70% glucose in thallus culture, supplementing the culture medium and resistance, and preparing L-hydroxyproline from L-hydroxyproline and L-hydroxyproline. 0.8% lactose is selected as an inducer and directly added for 11 h, CCTC is adopted as a surfactant, immobilized cells are selected, a pilot tank is utilized for conversion, and substrates L-proline and alpha-ketoglutaric acid are fed into the system to adjust the pH of the system and remove MES and Tris, so that the ion concentration of the system is greatly r