Kanamycin degrading enzyme AquKGD gamma gene and application thereof

• Main Authors: CHEN LIWEN, CHEN BILIAN, FANG SENHAI, WANG MINGZI, CHEN ZHIHONG
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS; CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING ORPROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; FIRE-FIGHTING; HUMAN NECESSITIES; LIFE-SAVING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINED SOIL SOIL; SPIRITS; TRANSPORTING; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; VINEGAR; WINE

The invention discloses a kanamycin degrading enzyme AquKGD gamma gene and an application of the kanamycin degrading enzyme AquKGD gamma gene. 1, and the nucleotide sequence of the AquKGD gamma gene is as shown in SEQ ID NO. 2. The AquKGD gamma can be used for degrading kanamycin (amino cyclic alcohol 4, 6-O-amino sugar diglycoside) into amino cyclic alcohol 6-O-amino glycoside. The product of kanamycin degraded by AquKGD gamma loses the bacteriostatic activity on bacteria, in addition, the cytotoxicity of the degradation product is evaluated, and the toxicity is obviously reduced compared with that of parent kanamycin with the same concentration. The result shows that when the AquKGD gamma is used for degrading and repairing kanamycin in the environment, secondary pollution cannot be caused, and application feasibility is achieved. 本发明公开了卡那霉素降解酶AquKGDγ基因及其应用。AquKGDγ基因的核酸序列如SEQ ID NO.2所示。AquKGDγ能将卡那霉素（氨基环醇4,6-O-氨基糖二苷）降解为氨基环醇6-O-氨基糖苷。卡那霉素经AquKGDγ降解后的产物失去了对细菌的抑菌活性，此外，对降解产物的细胞毒性进行评估，发现与同浓度的母体卡那霉素相比，毒性显著降低。该结果表明用