Method for inducing osteogenic differentiation of stem cells

• Main Authors: ZENG YE, YAO XINGHONG, SHEN JUNYI
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for inducing osteogenic differentiation of stem cells. The method comprises the following steps: inducing the osteogenic differentiation of the stem cells through hydrogel with a plurality of regular polygon micropatterns, wherein the side length of the regular polygons is greater than or equal to the short axis length of the stem cells, and the distance between two adjacent regular polygons is smaller than or equal to the short axis length of the stem cells The stem cell osteogenic differentiation can be effectively induced by adopting the method disclosed by the invention, the cell growth is not required to be limited to form micro-pattern morphology in the differentiation process, a chemical inducer is not required to be added, and the effect of inducing the osteogenic differentiation of the stem cells by adopting the method disclosed by the invention is more excellent than that of the chemical inducer. 本发明公开了一种诱导干细胞成骨分化的方法，该方法包括以下步骤：通过具有多个正多边形微图案的水凝胶诱导干细胞成骨分化；其中，正多边形边长大于等于