Detection method of aflatoxin B1 based on NH2-MIL-53 (Al)

• Main Authors: GE JIACHENG, YANG QINGLI, ZHANG FENGYAN, MA YONGCHAO, SUN RUIQING, HOU XIUDAN
• Format: Patent
• Language: Chinese; English
• Published: 10.10.2023
• Subjects: BRAIDING; CHEMISTRY; COMPOSITIONS BASED THEREON; COTTON-WOOL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS,NON-WOVEN FABRICS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; KNITTING; LACE-MAKING; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARYMATERIAL; MEASURING; METALLURGY; NON-WOVEN FABRICS; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; TESTING; TEXTILES; THEIR PREPARATION OR CHEMICAL WORKING-UP; TRIMMINGS; WADDING

The invention discloses a method for detecting aflatoxin B1 based on NH2-MIL-53 (Al), and belongs to the technical field of aflatoxin detection. The NH2-MIL-53 (Al) is a yellow solid obtained by dissolving AlCl3. 6H2O in ultrapure water, adding NH2-BDC while stirring, then adding a urea aqueous solution while stirring, keeping at 150 DEG C for 5 hours, and naturally cooling to room temperature. The prepared NH2-MIL-53 (Al) has good sensitivity and selectivity to AFB1, and the portable flexible sensor is prepared by loading a fluorescent probe on the electrostatic spinning nanofiber film. The successful combination of the flexible sensor and the smart phone greatly reduces the detection cost and time, and provides a promising method for the qualitative identification and quantitative detection of the field AFB1. 本发明公开了一种基于NH2-MIL-53(Al)的黄曲霉毒素B1的检测方法，属于黄曲霉毒素检测技术领域。本发明的NH2-MIL-53(Al)是由AlCl3·6H2O溶于超纯水中，在搅拌下加入NH2-BDC，然后在搅拌条件下加入尿素水溶液，在150℃保持5h，自然冷却至室温，得到的黄色固体。本发明制备的NH2-MIL-53(Al)对AFB1具有较好的灵敏度和选择性，通过在静电纺丝纳米纤维薄膜上加载荧光