Engineered ssDNA enzyme-free CRISPR endonuclease

• Main Authors: GARVEY, GLEN, S, KRIEGER ELYSIA K
• Format: Patent
• Language: Chinese; English
• Published: 03.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure provides compositions related to engineered CRISPR endonuclease proteins having a reduced ability to non-specifically cleave single stranded DNA (ssDNA) as compared to a protein of its reference wild type. The present disclosure also provides methods related to the use and production of engineered CRISPR endonuclease proteins having a reduced ability to non-specifically cleave ssDNA as compared to proteins of its reference wild type. 本公开提供了与工程化CRISPR内切核酸酶蛋白相关的组合物，所述蛋白与其参照野生型的蛋白相比具有降低的非特异性切割单链DNA(ssDNA)的能力。本公开还提供了与工程化CRISPR内切核酸酶蛋白的使用和产生相关的方法，所述蛋白与其参照野生型的蛋白相比具有降低的非特异性切割ssDNA的能力。