Method for improving flux of interaction experiment of compound and protein

• Main Authors: CHEN SHUNXING, JI HONGCHAO
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for improving compound and protein interaction experiment flux, which comprises the following steps of: forming a plurality of mixture systems by using a plurality of compounds to be detected according to a certain mixing rule, and establishing a corresponding relationship between the interaction capability of each compound to be detected and target protein and the mixture systems; and target protein corresponding to the compound to be detected is analyzed in a high-flux manner. According to the analytical method, the experimental detection flux of the existing compound-target protein to be detected can be improved by more than 10 times, meanwhile, the experimental cost and time are saved by more than 90%, the manpower, time and experimental consumable cost are greatly reduced, and the analytical method has remarkable economic significance. 本申请公开了一种提高化合物与蛋白质相互作用实验通量的方法，本申请的方法采用将多个待测化合物按一定混合规则组成多个混合物体系，并建立起每个待测化合物与靶点蛋白的作用能力与混合物体系之间的对应关系，进而高通量的解析出待测化合物相对应的靶点蛋白。本申请的解析方法可将现有待测化合物-