Method for measuring IRAK4 expression quantity

• Main Authors: PENG ZHANGXIAO, FANG HOUQIN, WANG ZEHAI, SHEN JIAXI, SHU LIEBO, WANG SHAN
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a sMRM targeted measurement method of interleukin-1 receptor associated kinase 4 (IRAK4) and application of the sMRM targeted measurement method. According to the method, the expression quantity of IRAK4 protein is represented by detecting the content of a symbolic peptide fragment (with uniqueness) of IRAK4; two independent symbolic peptide fragment sequences of the IRAK4 are GYVNNTTVAVK and TANTLPSK. The method comprises the following steps: pretreatment of cell sample protein, sMRM method development, and sMRM detection and data analysis of the IRAK4 in a clinical cell sample. The method can be used for evaluating the degradation amount, namely the drug effect, of various forms of drugs such as PROTAC and a small-molecule inhibitor developed by taking IRAK4 as a drug target to IRAK4 protein; the content of IRAK4 protein in various types of biological samples such as cells/plasma is quantified to assist diagnosis, detection, disease course monitoring and prognosis evaluation of infla