Application of molecular marker combination in detection of seed purity of watermelon variety Sumi 518

• Main Authors: HOU QIAN, LIU JINQIU, XU JIAN, ZHANG MAN, XU JINHUA, ZHU LINGLI, YAO XIEFENG, YANG XINGPING, LOU LINA, LIU GUANG
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses application of a molecular marker combination in detecting the seed purity of a watermelon variety 'Sumi 518'. Based on simplified genome sequencing results of male and female parents of a watermelon variety 'Sumi 518', two SNP markers suitable for purity identification of 'Sumi 518' seeds are screened, and two primer combinations for detecting the SNP markers are designed. By utilizing the two SNP markers, high-throughput and high-efficiency purity identification of the Sumi 518 seeds can be realized, the defects of time and labor waste in field identification are overcome, and a powerful technical support is provided for guaranteeing the quality of the seeds of the watermelon variety Sumi 518. 本发明公开了分子标记组合在检测西瓜品种'苏蜜518'种子纯度中的应用。本发明基于西瓜品种'苏蜜518'父、母本简化基因组测序结果，筛选出适用于'苏蜜518'种子纯度鉴定的2个SNP标记，并设计出用于检测SNP标记的2个引物组合。利用这2个SNP标记，可实现'苏蜜518'种子的高通量、高效率纯度鉴定，克服田间鉴定费时、费工的缺点，为保障西瓜品种'苏蜜518'的种子质量提供强有力的技术支撑。