Primer specific sites of high-reproduction gene FecB of Chalafrom sheep and application of primer specific sites

• Main Authors: HAO ZHIGUO, ZHANG HECHUN, YANG SHUHUAN, YUE MIJIANG, HOU SIBING, LI JIAQI, CONG YUYAN, WANG XIAOWEI, ZHANG HONGLIANG, SUN YINGGANG, LI XI, PAN YUAN, ZHANG QIU, QIAO YANJUN, XIA DECUI, WANG GUOCHUN, WANG CHENG, WU YANZHI, LI SHUAITONG, WANG ZEYING, LIU QINGKUN, WU DONGLEI, LIU YINING, KONG LINGCHAO, LI SIYI, CHEN RUI, ZHANG YANPING
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of reproductive function gene detection, in particular to a primer specific site of a chalalei hyperreproductive gene FecB and application thereof.The method comprises the steps that a chalalei blood sample is collected, specifically, the blood sample with DNA extracted is 1 ml, and after blood sampling, the blood sample is put into a blood sampling tube containing EDTA and stored at the temperature of-20 DEG C; dNA is extracted, wherein an Ezup column type blood genome DNA extraction kit is used for DNA extraction, and preservation is conducted at the temperature of-20 DEG C; and reproductive performance data: the lambing data are acquired and statistically analyzed by the same sheep farm. According to the invention, SNP is utilized to perform genetic typing on the FecB gene of the Chalalair sheep, an SNP site is found, genetic diversity analysis is performed on the SNP site, correlation analysis is performed on the SNP site and reproductive performance, and a gen