3D biological printing ink for cell culture meat and method for preparing cell culture scaffold

• Main Authors: XU ENBO, LIU DONGHONG, YIN JUN, CHEN QIHE, XIN QIPU, NIU RUIHAO, CHEN MINXUAN, ZHU YANG, CHEN JUN
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: ADDITIVE MANUFACTURING TECHNOLOGY; ADDITIVE MANUFACTURING, i.e. MANUFACTURING OFTHREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVEAGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING,STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; GENERAL PROCESSES OF COMPOUNDING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; TRANSPORTING; VINEGAR; WINE; WORKING-UP

The invention provides 3D bio-printing ink and a method for preparing a cell culture scaffold, the 3D bio-printing ink takes nano starch as a functional factor, the 3D printing property of hydrogel under a low temperature condition is greatly enhanced, and adherent proliferation of fish myoblasts is greatly promoted. Compared with a common gelatin scaffold, the cell culture scaffold obtained by printing based on the ink can effectively promote wall attachment and proliferation of myoblasts. The nano starch, the gelatin and the sodium alginate are all food-grade raw materials, and have great application prospects in the field of meat culture. 本发明提供一种3D生物打印墨水及制备细胞培养支架的方法，其中，3D生物打印墨水以纳米淀粉作为功能性因子，大幅度增强低温条件下水凝胶的3D打印性质，并大幅促进鱼成肌细胞贴壁增殖。基于该墨水打印得到的细胞培养支架，相较于普通明胶支架，可以有效促进成肌细胞贴壁和增殖。本发明所属纳米淀粉、明胶、海藻酸钠均为食品级原料，在培养肉领域应用前景大。