Anti-human CD47 protein antibody, nucleic acid molecule and application thereof

• Main Authors: YAN ZHENG, LI HUDAN, FU KANG
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an anti-human CD47 protein antibody, a nucleic acid molecule and application thereof, and relates to the technical field of CD47 antibody preparation. The provided antibody for resisting the human CD47 protein has relatively high affinity with natural antigen (CD47) protein, is a mouse-derived monoclonal antibody, and can specifically recognize the CD47 protein expressed in cells such as human cell lines MD-MB-231 (breast cancer cells) and Jurkat and the like. Therefore, the human CD47 protein can be used for developing a detection or diagnosis reagent and a kit which take the human CD47 protein as a diagnosis marker. The anti-CD47 antibody does not cause agglutination of human red blood cells at a certain concentration. The method can be used for further humanized transformation, so that targeted drug delivery is realized. In addition, the anti-CD47 antibody provided by the invention can effectively inhibit proliferation of tumor cells such as leukemia cells and breast cancer cells, a