IVK8-RGD polypeptide, preparation method of hydrogel of IVK8-RGD polypeptide, and composition loaded with human umbilical cord-derived mesenchymal stem cells

• Main Authors: DING XIN, NIU WENJING, LYU XUE, ZHAO XIAOMIAO
• Format: Patent
• Language: Chinese; English
• Published: 22.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an IVK8-RGD polypeptide and a preparation method of a hydrogel thereof, a composition loaded with human umbilical cord-derived mesenchymal stem cells, the IVK8-RGD polypeptide, a self-assembled polypeptide IVK8 with eight amino acids IEVEIRVK is subjected to functional modification by using a bioactive sequence RGD, and the hydrogel of the IVK8-RGD polypeptide is obtained. Glycine and aspartic acid are selected as linking groups of the bioactive sequence RGD and the self-assembled polypeptide IVK8, charges of the whole peptide chain are in a--+ + arrangement mode, the self-assembled gel can be formed by incubation for 15 minutes at 37 DEG C, and adjustable mechanical strength and long-term stability are realized. The IVK8-RGD polypeptide hydrogel loaded with human umbilical cord source mesenchymal stem cells can effectively inhibit excessive fibrosis of endometrium, relieve inflammatory response, promote proliferation of endometrium and vascular remodeling, promote repair of endometriu