Method for identifying and monitoring interaction between protein and ligand

• Main Author: CHEN SHUNXING
• Format: Patent
• Language: Chinese; English
• Published: 19.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention relates generally to the field of biochemistry. In particular, the present invention relates to a method of detecting or determining a target bound to a ligand in a sample, the method comprising contacting a sample comprising one or more cells with a cell permeable denaturing agent to promote intracellular unfolding of the target in the sample, and then lysing the sample. The level of non-aggregating targets or aggregating targets in the lysed sample is then detected or determined, where the difference in the level of non-aggregating targets or aggregating targets as compared to a reference indicates the presence or level of ligand-binding targets in the sample. In particular embodiments, the cell permeable denaturant is urea or a derivative thereof. Also provided are methods of identifying candidate ligands or predicting the efficacy of a drug in a subject. 本发明总体上涉及生物化学领域。特别地，本发明涉及检测或测定与样品中配体结合的靶标的方法，所述方法包括使包含一种或多种细胞的样品与细胞渗透性变性剂接触，以促进样品中靶标的细胞内去折叠，然后裂解样品。然后检测或测定裂解样品的非聚集性靶标或聚集性靶标的水平，其中与参考