METHODS AND COMPOSITIONS FOR PREPARING AMIDE COMPOUNDS

• Main Authors: NAGARAJAN HARISH, ZHANG BO, BAHAN, SUDHAKAR, GHATAK SUDIP, YOUNG TERENCE H, SHAH ANKUR, SHAH JIGNESH, KHODAYARI AMIN, ECKLI NICOLAS
• Format: Patent
• Language: Chinese; English
• Published: 19.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed are biosynthesis methods and engineered microorganisms that enhance or improve the biosynthesis of 6-aminohexanoate, hexamethylenediamine, hexanoic acid, caprolactone, or caprolactam. The engineered microorganisms are modified to include, for example, an up-regulated and/or exogenous transporter of 6-aminocaproate, a deletion and/or down-regulated input of 6-aminocaproate, an up-regulated and/or exogenous glutamate dehydrogenase, and/or a deletion and/or down-regulation of rcsA and/or cpsBG. Other engineered microorganisms may have a disruption of the endogenous transporter of the 6-aminocaproate. 本发明公开生物合成方法和经工程改造的微生物，其增强或改良6-氨基己酸酯、己二胺、己酸、己内酯或己内酰胺的生物合成。所述经工程改造的微生物被修饰成包括例如6-氨基己酸酯的上调的和/或外源转运体、6-氨基己酸酯的缺失和/或下调的输入物、上调的和/或外源的谷氨酸脱氢酶，以及/或rcsA和/或cpsBG的缺失和/或下调。其它经工程改造的微生物可以具有6-氨基己酸酯的内源性转运体的破坏。