Composition and kit for detecting mycoplasma

• Main Authors: XIONG QIYAN, WANG LI, WANG JIA, LIU BEIBEI, WEI YANNA, YU YANFEI, WU YUZI, LIU MAOJUN, SHAO GUOQING, FENG ZHIXIN
• Format: Patent
• Language: Chinese; English
• Published: 19.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a composition and a kit for detecting mycoplasma, and belongs to the technical field of biology. The composition for detecting mycoplasma is characterized in that the composition is an aqueous solution containing a primer M-F, a primer M-R and a probe M-P; the sequence of the M-F is as shown in SEQ ID NO: 1; the sequence of M-R is as shown in SEQ ID NO: 2; the nucleotide sequence of the probe M-P is shown as SEQ ID NO.3, the 5'end of the probe M-P is connected with a fluorophore FAM, and the 3 'end of the probe M-P is connected with a quenching group BHQ1. When the composition is used for detecting mycoplasmas, the sensitivity is high, the specificity is strong, and the detection range is wide. 本发明的提供用于检测支原体的组合物和试剂盒，属于生物技术领域。用于检测支原体的组合物，其特征在于是含有引物M-F、引物M-R和探针M-P的水溶液；所述M-F的序列如SEQ ID NO:1所示；M-R的序列如SEQ ID NO:2所示；探针M-P的核苷酸序列如SEQ ID NO:3所示，5'端连接有荧光基团FAM，3'端连接有淬灭基团BHQ1。采用本发明组合物检测支原体，灵敏度高、特异性强、检测范围广。