Grape cytokinin response regulatory factor gene VvRR1 and application thereof

• Main Authors: ZHANG HONGYAN, CHEN KEQIN, ZHANG SHIHAO, FANG YULIN, ZHANG KEKUN, SUN JIAHUA
• Format: Patent
• Language: Chinese; English
• Published: 19.09.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a grape cytokinin response regulatory factor gene VvRR1 and application of the grape cytokinin response regulatory factor gene VvRR1. The whole length of the disclosed gene sequence is 1935 bp, 681 amino acids are encoded, an overexpression vector and a related gene silencing vector are constructed, PRI101-VvRR1 (gene overexpression vector) and TRV-VvRR1 (gene silencing vector) are instantaneously transformed in cabernet sauvignon fruits, PRI101-VvRR1 and RNAi-VvRR1 (gene silencing vector) are stably transformed in grape calluses, the expression quantity of related genes and the tannin content are detected, and the tannin content is detected. And verifying the function of the VvRR1 in the grape tannin synthesis process. Experiments prove that the overexpression of VvRR1 in grapes obviously improves the expression of VvRR1 in the protein level and the expression quantity of a tannin synthesis key gene VvANR, and the tannin content is also obviously increased; the transcription factor VvR