Method for classifying white blood cells into sub-groups

• Main Author: TORIYA YUJI
• Format: Patent
• Language: Chinese; English
• Published: 19.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The purpose of the present invention is to provide a method for classifying and counting basophilic granulocytes with high accuracy even when a test specimen that has elapsed since collection is used in a method for classifying leukocytes into subgroups. The problem is solved by a method comprising preparing a measurement sample by mixing a specimen containing leukocytes, a hemolytic reagent containing a surfactant, a first fluorescent dye, and a second fluorescent dye, irradiating particles in the measurement sample with light, detecting first fluorescence information containing fluorescence from the first fluorescent dye, and detecting the first fluorescence information based on the fluorescence from the second fluorescent dye. On the basis of optical information of scattered light information and second fluorescence information of fluorescence from the second fluorescent dye, a cell group containing basophils is screened from the particles in the measurement sample on the basis of optical information inclu