Tobacco yellowing regulation gene NtRALF08, protein, polypeptide, yellowing accelerant, preparation method and application

• Main Authors: ZHANG QINGFU, PENG SHUGUANG, LI LIANGYONG, LI YUHUI, ZHAI ZHENGGUANG, PAN RUIPENG, GUO LIANG, HE MINGJUN, XIE PENGFEI, ZHOU HONG, DUAN MEIZHEN
• Format: Patent
• Language: Chinese; English
• Published: 15.09.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a tobacco yellowing regulation gene NtRALF08, a protein, a polypeptide, a yellowing accelerant, a preparation method and application. The application discovers that the NtRALF08 gene participates in and regulates tobacco aging and yellowing for the first time, and an analysis result shows that the NtRALF08 polypeptide artificially synthesized by the NtRALF08 gene and the prepared tobacco yellowing accelerant can promote tobacco aging and yellowing, the application effect is remarkable, and biological safety is achieved. Meanwhile, the tobacco yellowing regulating polypeptide disclosed by the invention can be artificially synthesized on a large scale, and is convenient to synthesize and obtain. The tobacco yellowing accelerant is simple in preparation and use method and high in operability, tobacco yellowing can be promoted in a large area, and the tobacco yellowing time can be regulated and controlled. 本发明提供了一种烟草落黄调控基因NtRALF08、蛋白、多肽、落黄促进剂、制备方法及应用。本申请首次发现NtRALF08基因参与、调控烟草衰老落黄，且分析结果表明由NtR