Engineered CRISPR-CAS proteins and methods of use thereof

• Main Authors: WATERS, JAMES, M, GUFFEY SARAH LYNN
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are engineered CRISPR-Cas proteins and methods of using such proteins. The present invention relates to a nuclease, in particular a V-type CRISPR-Cas nuclease, such as Cas12a, having a nuclease or nickase domain from a non-V-type CRISPR-Cas nuclease inserted into an inter-domain header region between the Rec1 and Rec2 domains. Also described herein are complexes, compositions, and systems comprising the engineered proteins of the invention, each of which can be used to modify or edit a target nucleic acid. The engineered proteins of the invention may be enzymes and/or may be RNA-guided DNA binding proteins. 本文描述了工程化CRISPR-Cas蛋白质和使用此类蛋白质的方法。特别是具有插入到Rec1和Rec2结构域之间的域间接头区域中的来自非V型CRISPR-Cas核酸酶的核酸酶或切口酶结构域的V型CRISPR-Cas核酸酶，如Cas12a。本文还描述了包括本发明的工程化蛋白质的复合物、组合物和系统，其各自可以用于修饰或编辑靶核酸。本发明的工程化蛋白质可以是酶和/或可以是RNA引导的DNA结合蛋白。