Method for detecting caffeic acid in selfheal seeds and application of method

• Main Authors: CHEN BIN, YUAN CHENG, CHEN HUOLIN, CHEN JIAO, ZENG YUCHENG, LI YING, CHENG CHANGKUI
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for detecting caffeic acid in selfheal seeds and application of the method. The detection method comprises the following steps: qualitatively identifying caffeic acid in selfheal seeds by utilizing a thin layer chromatography; the method comprises the following steps: directly extracting caffeic acid by using a specific polar solvent extraction method, separating the caffeic acid from fat-soluble components, comparing with a caffeic acid reference substance, and measuring the content of the caffeic acid in the selfheal seeds by using a high performance liquid chromatography. The detection method has the advantages of being high in accuracy and precision, good in stability and repeatability and the like, the method for detecting the quality of the caffeic acid component in development and utilization of the selfheal seeds is provided, and high specificity and good reproducibility are achieved. 本发明公开了一种夏枯草籽中咖啡酸的检测方法及其应用。该检测方法包括利用薄层色谱法对夏枯草籽中咖啡酸进行定性鉴别；利用特定的极性溶剂提取法直接提取咖啡酸并与脂溶性成分分离，