SNP (Single Nucleotide Polymorphism) site related to tomato brown wrinkled fruit virus resistance, TBR11 gene and application

• Main Authors: JIA ZHIQI, QI JIEYU, LI JISUO, KANG DONGMU, ZHANG YAN, LI YING, WANG PANQIAO
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to tomato brown wrinkled fruit virus resistance, a TBR11 gene and application. The SNP site can lay a foundation for finally realizing cloning of the TBR11 gene and further researching a molecular mechanism of the brown wrinkled fruit virus resistance. On the other hand, the SNP locus is closely linked with the TBR11 gene, so that the SNP locus can be directly used for molecular marker assisted breeding of the tomato brown wrinkled fruit virus resistant material, and the molecular marker has the advantages of simplicity, convenience, rapidness and high throughput in an assisted breeding system; therefore, the molecular marker provided by the invention has better application value in the cultivation of new varieties resistant to the brown wrinkled fruit virus. The function of the TBR11 gene is further verified in a mode of silencing the TBR11 gene, the result shows that the TBR11 gene is a gene endowed with ToBRFV resistance, and the