Production of closed linear DNA

• Main Authors: ROTHWELL PAUL, PORTER NEIL, ADIE THOMAS
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to the production of closed linear DNA, in particular to an improved method for the production of closed linear deoxyribonucleic acids (DNAs), in particular for the enzymatic production of closed linear DNA molecules without cells, preferably using closed linear DNA as a template for DNA synthesis. The invention also relates to novel closed linear DNA species suitable for use as a template in an improved method for producing closed linear DNA. In addition, the invention relates to an intermediate product of said method, as a result of this enabling the number of closed linear DNA produced by the template to be greater than that of methods known in the prior art. 本发明涉及闭合线性DNA的生产，具体而言，本发明涉及用于生产闭合线性脱氧核糖核酸(DNA)的改良方法，尤其是用于在无细胞下酶促生产闭合线性DNA分子，优选使用闭合线性DNA作为DNA合成的模板。本发明还涉及新型的闭合线性DNA物质，其适合作为模板用在生产闭合线性DNA的改良方法中。另外，本发明还涉及所述方法的中间产物，原因是这能够使由模板生产的闭合线性DNA的数量大于现有技术已知的方法。